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KMID : 0861020100250040055
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Korea Journal of Herbology
2010 Volume.25 No. 4 p.55 ~ p.59
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Antioxidant Activities and Inhibitory Effect on Oxidative DNA Damage of Nelumbinis Semen Extracts
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Park Jae-Ho
Lee Byung-Gu
Byun Gwang-In
Kim Do-Wan
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Abstract
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Objective: This study was conducted to investigate the antioxidant activity and inhibitory effect on oxidative DNA damage of Nelumbinis Semen Extracts
Methods: Nelumbins semen were extracted with hot-water and ethylacetate (EtOAC). The 1,1-diphenyl-2- picrylhydrazyl (DPPH) and hydroxyl radical scavenging assay and Fe2+ chelating assay were performed for antioxidative effect and φX-174 RF I DNA cleavage assay and intracellular DNA damage assay were used for inhibitory effect on intracellular DNA damage.
Results: In DPPH, Hydroxyl radical scavenging activity and Fe2+ chelating activity of EtOAC extracts were 96.22%, 53.53%, 64.72%, while those of hot-water extracts were 20.86%, 10.72%, 29.74% at 200 ㎍/㎖, respectively. In φX-174 RF I plasmid DNA cleavage assay, the protective effects of EtOAC and hot-water extracts against oxidative DNA damage were 76% and 6% at 200 ㎍/㎖, respectively.
Conclusion: These results indicated that the seed extracts of Nelumbo nucifera can be used as a natural antioxidants, which effectively inhibits the oxidative DNA damage.
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KEYWORD
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Nelumbinis Semen, DPPH, Hydroxyl radical, Fe2+ chelating, DNA cleavage, DNA migration
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